Reference channel registration

reference_registration.reference_registration()

This script is used to register the stitched reference channels for the processed hybridization. The comparison is sequential (from Hyb1–>HybN) and not all the hybridization steps are required.

The output are pickle files with the recalculated corners according to the registration

The input parameters are entered via argparse

path: string.

Exact path to the folder with the stitched .sf.hdf5

reference_gene: string.

Reference gene used for stitching

fraction: float.

Fraction of the image to use for the registration. Selection start from the center of the image. Default 0.2